NAME

       samtools-collate - shuffles and groups reads together by their names

SYNOPSIS

       samtools collate [options] in.sam|in.bam|in.cram [<prefix>]

DESCRIPTION

       Shuffles  and  groups  reads  together  by  their names.  A faster alternative to a full query name sort,
       collate ensures that reads of the same name are grouped together in contiguous groups, but  doesn't  make
       any guarantees about the order of read names between groups.

       The  output  from this command should be suitable for any operation that requires all reads from the same
       template to be grouped together.

       If present, <prefix> is used to name the temporary files that collate uses when  sorting  the  data.   If
       neither  the  '-O' nor '-o' options are used, <prefix> must be present and collate will use it to make an
       output file name by appending a suffix depending on the format written (.bam by default).

       If either the -O or -o option is used, <prefix> is optional.  If <prefix> is absent, collate  will  write
       the temporary files to a system-dependent location (/tmp on UNIX).

       Using  -f for fast mode will output only primary alignments that have either the READ1 or READ2 flags set
       (but not both).  Any other alignment records  will  be  filtered  out.   The  collation  will  only  work
       correctly if there are no more than two reads for any given QNAME after filtering.

       Fast  mode keeps a buffer of alignments in memory so that it can write out most pairs as soon as they are
       found instead of storing them in temporary files.  This allows collate to avoid some work and  so  finish
       more  quickly  compared  to  the  standard  mode.  The number of alignments held can be changed using -r,
       storing more alignments uses more memory but increases the number of pairs that can be written early.

       While collate normally randomises the ordering of read pairs, fast  mode  does  not.   Position-dependent
       biases  that  would  normally  be broken up can remain in the fast collate output.  It is therefore not a
       good idea to use fast mode when preparing data for programs that expect randomly  ordered  paired  reads.
       For  example  using fast collate instead of the standard mode may lead to significantly different results
       from aligners that estimate library insert sizes on batches of reads.

OPTIONS

       -O      Output to stdout.  This option cannot be used with '-o'.

       -o FILE Write output to FILE.  This option cannot be used with '-O'.

       -u      Write uncompressed BAM output

       -l INT  Compression level.  [1]

       -n INT  Number of temporary files to use.  [64]

       -f      Fast mode (primary alignments only).

       -r INT  Number of reads to store in memory (for use with -f).  [10000]

       --no-PG Do not add a @PG line to the header of the output file.

       -@, --threads INT
               Number of input/output compression threads to use in addition to main thread [0].

AUTHOR

       Written by Heng Li from the Sanger Institute and extended by Andrew Whitwham.

SEE ALSO

       samtools(1), samtools-sort(1)

       Samtools website: <http://www.htslib.org/>

samtools-1.13                                      7 July 2021                               samtools-collate(1)